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Taq T DNA Polymerase
Taq DNA Polymerase modified (taq-T), is a modified form of the enzyme from the
thermophilic eubacterium Thermus aquaticus. This enzyme has a modification in
dNTP binding site. Due to the modification enzyme incorporates dUTP and
dideoxynucleotides more efficiently in comparison with Taq DNA Polymerase. Enzyme
may be used for cycle sequencing, recommended for dUTP/bio-dUTP incorporation
reactions
Storage and dilution buffer:
10 mM K-phosphate bufffer, pH 7.4, 100 mM KCl, 0.1 mM EDTA, 50% glycerol,
0.1% Triton X-100, 0.1% Tween-20.
Reaction buffer (x10) incomplete:
160 mM (NH4)2SO4, 670 mM TrisHCl pH 8.8, 0,1% Tween-20, plus one tube
plus one tube MgCl2 (100 mM)
Reaction buffer (x10) complete:
160 mM (NH4)2SO4, 670 mM TrisHCl pH 8.8, 0,1% Tween-20, 25 mM MgCl2
Reaction buffer (x10) complete II KCl:
500 mM KCl, 100 mM TrisHCl pH 8.8, 0,1% Tween-20, 15 mM MgCl2
Recommended MgCl2 concentration: 1.5 mM - 6 mM
Concentration: 5 units/ml
| Anexo | Tamanho |
|---|---|
| Taq-T_DNA_Polymerase.pdf | 58.37 KB |
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