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GC-Rich PCR DNA Polymerase
GC Rich PCR DNA Polymerase is a high fidelity thermostable polymerase that
amplifies target DNA up to 6 kbp in lenght with superior accuracy and yield.
The enzyme´s 3´->5´exonuclease dependent proofreading activity results in a lower
PCR mutation frequency than other commercially available DNA Polymerase
The elongation rate and processivity are 5 times and 10 to 15 times higher,
respectively, than for Pfu DNA Polymerase, resulting in highly accurate and robust
yields in short reaction times. The enzyme has no 5-->3´exonuclease activity and
generates blunt-ended PCR products suitable for cloning
amplifies target DNA up to 6 kbp in lenght with superior accuracy and yield.
The enzyme´s 3´->5´exonuclease dependent proofreading activity results in a lower
PCR mutation frequency than other commercially available DNA Polymerase
The elongation rate and processivity are 5 times and 10 to 15 times higher,
respectively, than for Pfu DNA Polymerase, resulting in highly accurate and robust
yields in short reaction times. The enzyme has no 5-->3´exonuclease activity and
generates blunt-ended PCR products suitable for cloning
Storage Buffer:
50 mM Tris-HCl (pH 8.0); 50 mM KCl; 1 mM DTT; 0.1 mM EDTA; stabilizers; and
50% (v/v) glycerol
50 mM Tris-HCl (pH 8.0); 50 mM KCl; 1 mM DTT; 0.1 mM EDTA; stabilizers; and
50% (v/v) glycerol
Reaction buffer GC-rich (10x):
1.2 M Tris-HCl (pH 8.0); 100 mM KCl; 60 mM (NH4)2SO4; 1% Triton X-100;
0.01% BSA. Plus an extra Tube of: 25 mM MgCl2
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