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Psp DNA Polymerase
Psp DNA Polymerase is a thermostable enzyme isolated from Pyrococcus species.
The enzyme is quite similar to Pfu DNA Polymerase, it replicates DNA at 75ºC,
catalyzing the polymerization of nucleotides into duplex DNA in the 5´-->3 direction.
Psp DNA Polymerase possesses 3´-->5´exonuclease activity. Nucleotide
misinsertions that may occur during polymerization are excised by the proofreading
activity of the enzyme. Psp DNA Polymerase is recommended for use in PCR and
primer extension reactions that require high-fidelity synthesis.
Concentration: 5000 units/ml
The enzyme is quite similar to Pfu DNA Polymerase, it replicates DNA at 75ºC,
catalyzing the polymerization of nucleotides into duplex DNA in the 5´-->3 direction.
Psp DNA Polymerase possesses 3´-->5´exonuclease activity. Nucleotide
misinsertions that may occur during polymerization are excised by the proofreading
activity of the enzyme. Psp DNA Polymerase is recommended for use in PCR and
primer extension reactions that require high-fidelity synthesis.
Concentration: 5000 units/ml
Supplied Buffers:
Incomplete (10x): 200 mM Tris-HCl (pH 8,8), 100 mM KCl, 100 mM
(NH4)2SO4, 1,0 % Triton X-100, 1 mg/ml nulease-free BSA ; supplied with 1
ml of 25mM MgSO4 separately packed
Complete (10x): 200 mM Tris-HCl (pH 8,8), 100 mM KCl, 100 mM (NH4)2SO4,
20 mM MgSO4, 1,0 % Triton X-100, 1 mg/ml nulease-free BSA
One tube with MgCl2 (100 mM)
One tube MgSO4 (25 mM)
| Anexo | Tamanho |
|---|---|
| Psp_DNA Polymerase.pdf | 44.23 KB |
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