Klein Taq DNA Polymerase

Klein Taq DNA Polymerase BIORON

KleinTaq is a derivative of Taq DNA polymerase. It is a

5'-exo-minus N-terminally truncated Thermus aquaticus DNA

polymerase. As expressed from a gene construct in E.coli,

translation initiates at Met236, bypassing the 5'-3' exonuclease

domain of the DNA polymerase-encoding gene.

The enzyme reveals a highly active and even more heat-stable DNA

polymerase activity in comparison with Taq DNA polymerase. The

optimal range of Mg²⁺ concentration for KleinTaq is broader than for

the majority of thermostable polymerases. This feature allows to

optimize reaction conditions easier then for other polymerases.

Repeated exposure to 98°C does not diminish the enzyme activity.

Significant activity remains even after exposure to 99°C.

Concentration: 5-10000 units/ml

Storage buffer:

10 mM K-phosphate pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM

DTT, 0.01% Tween 20, 50% glycerol

Reaction buffer 10x incomplete:

160 mM (NH₄)₂SO₄; 670 mM Tris-HCl pH (ph 8,8) ; 0.1%

Tween-20

Reaction buffer 10x complete:

160 mM (NH₄)₂SO₄; 670 mM Tris-HCl pH (ph 8,8) ; 0.1%

Tween-20, 25 mM MgCl₂

Reaction buffer 10x complete II KCl:

500 mM KCl; 100 mM Tris-HCl pH (ph 8,8) ; 0.1% Tween-20, 15

mM MgCl₂

Plus one tube MgCl2 (100mM)

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Klein Taq DNA Polymerase