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Tth DNA Polymerase
Tth DNA Polymerase is a thermostable enzyme of approximately
94 kDa isolated from eubacterium Thermus thermophilus strian HB8.
This enzyme replicates DNA at 74ºC .
It reveals RNA-dependent DNA-polymerase activity in the presence
of Mn2+ions.
The concentration of RNA template for effective reverse
transcription with Tth DNA polymerase should be higher if to
compare with reverse transcription directed by Reverse
Transcriptases (M-MuLV-AMV)
Concentration: 5000units/ml
94 kDa isolated from eubacterium Thermus thermophilus strian HB8.
This enzyme replicates DNA at 74ºC .
It reveals RNA-dependent DNA-polymerase activity in the presence
of Mn2+ions.
The concentration of RNA template for effective reverse
transcription with Tth DNA polymerase should be higher if to
compare with reverse transcription directed by Reverse
Transcriptases (M-MuLV-AMV)
Concentration: 5000units/ml
Storage buffer: 10 TrisHCl, pH 7.5, 300 mM KCl, 0.1 mM EDTA, 1
mM DTT, 50% glycerol.
Supplied Reaction buffers:
- RT-PCR buffer, one-step reaction (5 x): 250 mM bicine/KOH,
pH 8.2 (25°C); 575 mM K-acetate, 40% glycerol (v/v)
- 10 x Reverse transcription buffer: 100 mM Tris-HCl (pH 8.9 at
25°C), 900 mM KCl
- 10 x Tth PCR buffer: 100 mM Tris-HCl (pH 8.9 at 25°C), 1M
KCl, 500 µg/ml BSA, 0.5% Tween 20 and 15 mM MgCl2
- Mn2+: 1 - 2 mM for RNA - dependent c-DNA synthesis.
(supplied as MnCl2 in a separate tube).
- Mg2+: 3-6 mM for PCR reaction (supplied as MgCl2 in a
separate tube).
- RT-PCR buffer, one-step reaction (5 x): 250 mM bicine/KOH,
pH 8.2 (25°C); 575 mM K-acetate, 40% glycerol (v/v)
- 10 x Reverse transcription buffer: 100 mM Tris-HCl (pH 8.9 at
25°C), 900 mM KCl
- 10 x Tth PCR buffer: 100 mM Tris-HCl (pH 8.9 at 25°C), 1M
KCl, 500 µg/ml BSA, 0.5% Tween 20 and 15 mM MgCl2
- Mn2+: 1 - 2 mM for RNA - dependent c-DNA synthesis.
(supplied as MnCl2 in a separate tube).
- Mg2+: 3-6 mM for PCR reaction (supplied as MgCl2 in a
separate tube).
| Anexo | Tamanho |
|---|---|
| Tth_DNA_Polymerase.pdf | 83.45 KB |
| Tth_User_guide.pdf | 46.61 KB |
»
- 144 leituras