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Taq Red DNA Polymerase
Taq Red DNA Polymerase is Taq DNA Polymerase with a chemically
attached chromogenic molecule. The enzyme is stained in red. The
stain facilitates the visual control of mixing the enzyme with other
components.
attached chromogenic molecule. The enzyme is stained in red. The
stain facilitates the visual control of mixing the enzyme with other
components.
The stain doesn´t influence the enzyme activity and other general
characteristics.
characteristics.
Concentration: 5000 units/ml
Storage buffer:
10 mM K-phosphate, pH 7.4, 0.1 mM EDTA, 50% glycerol, 0.1%
Triton X-100, 0.1% Tween 20.
10 mM K-phosphate, pH 7.4, 0.1 mM EDTA, 50% glycerol, 0.1%
Triton X-100, 0.1% Tween 20.
Reaction buffer (x10) incomplete:
160 mM (NH4)2SO4, 670 mM TrisHCl pH 8.8, 0,1% Tween-20, plus
one tube MgCl2 (100 mM)
Reaction buffer (x10) complete:
160 mM (NH4)2SO4, 670 mM TrisHCl pH 8.8, 0,1% Tween-20, 25 mM
MgCl2
Reaction buffer (x10) complete II KCl:
500 mM KCl, 100 mM TrisHCl pH 8.8, 0,1% Tween-20, 15 mM MgCl2
| Anexo | Tamanho |
|---|---|
| TOP-Taq DNA Polymerase.pdf | 62.31 KB |
»
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